FLIM/FCS time-resolved spatial resolution microscopy system:

ISS launches a new generation of fast fluorescence lifetime imaging system FLIM/PLIM. The imaging speed can reach 20 fps (@ 256 × 256),Free choice of 1 × 1 to 4096 × 4096 pixel resolution;Simultaneously obtaining fluorescence lifetime imaging and confocal intensity imaging data to maintain single molecule level detection sensitivity.

Used in disciplines such as chemistry, nanotechnology, energy, biology, etc., for studying the mechanisms of single-molecule, living cell, micro area imaging, morphology, energy level structure, and energy transfer characteristics. Meet the lifetime imaging testing of upconversion quantum dots and related materials..

ISS takes the whole machine's fluorescence lifetime imaging system as its responsibility, realizing the perfect combination of confocal 3D scanning module (pinhole, 2D galvanometer, piezoelectric station or automatic worktable) and time-resolved module, providing full time domain fluorescence lifetime detection of<100ps-100ms; At the same time, the software integrates Phasor Plots fluorescence lifetime direct reading half circle compass vector graph technology, providing visual and intuitive fluorescence lifetime distribution and values.

Fluorescence lifetime imaging data analysis entersThe era of direct reading.

The ISS laser confocal scanning fluorescence lifetime imaging system can also meet the following special needs simultaneously:

1. Fluorescence lifetime of two-photon FLIM/PLIM imaging;

2. Fluorescence lifetime FLIM/PLIM imaging under deep ultraviolet excitation;

3. FLIM/PLIM imaging of fluorescence lifetime in the red second region;

4. Laser scanning large field of view live imaging FLIM/PLIM;

5. Spectral acquisition and spectral imaging;

6. AFM combination - live cell workstation combination - freezing and heating workstation combination;

7. Three dimensional tracking of nanoparticles; (proprietary technology)

Main function description: (Two photon function can be selected)

1. Laser confocal microscopyFluorescence intensity imagingLCM；

2. YingOptical lifetime imagingFLIM， Phosphorescence lifetime imaging PLIM;

3. Upconversion fluorescence(Lifetime) imaging, rare earth luminescence (lifetime) imaging, delayed fluorescence (lifetime) imaging;

4. Fluorescence fluctuation imaging FFS (FCS, FCCS, PCH, N&B, RICS, FLCS, scan FCS),

5. FLIM-FRET imagingFluorescence quantitative imaging;

6. Single quantum dot luminescence (lifetime) imaging, single-molecule and single-molecule fluorescence resonance transfer imaging (smFRET), including alternating excitation PIE imaging;

7. Steady state and transient polarization imaging;

8. Micro area fluorescence spectroscopy collection at 400-1100nm;

9. Anti beam testing (including professional software);
   

10. Upgrade of live cell workstation (including porous plate)）

    Instrument features:

• Real time direct reading to obtain fluorescence lifetime values and trends, as well as FRET efficiency distribution;

• choice350nm-1100nm plus 900nm-1700nmWavelength range detector, 2-4Channel detector, used for imaging, FLIM-FRET；

• Upgraded without wavelength interferenceAFM (upright or inverted), achieve the same regional morphology andFLIMSynchronous testing;

• Ultraviolet-so-Infrared excitation wavelength, single wavelength or supercontinuum laser; Single photon or two-photon lasers；

Main technical indicators

1. Fluorescence lifetime testing range: 100ps-100ms;

2. Minimum time resolution ≤ 1ps;

3. Data counting rate: 65 MHz/channel

4. Detection channel: upto 8 channels；

5. Equipped with XY galvanometer scanning, 5kHz scanning frequency, and XY closed-loop automatic table to achieve large-scale scanning;

6. Phasor plots are used for data analysis;

7. Spectral acquisition; 400-1100nm

8. Scanning transmission imaging;

9. Interface focusing system;

10. Variable temperature accessories; 77k-500k；